Banca de QUALIFICAÇÃO: RODOLFO RODRIGUES NUNES DA SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE: RODOLFO RODRIGUES NUNES DA SILVA
DATA : 10/07/2023
LOCAL: Defesa remota
TÍTULO:

Multidimensional hyperspectral microscopy and excitation spectroscopy for thermometry

PALAVRAS-CHAVES:

hyperspectral images, 3D localization, trivalent lanthanide ions, primary luminescent thermometers.

PÁGINAS: 122
RESUMO:

Hyperspectral microscopy and remote sensing via ratiometric luminescence thermometry are both innovative techniques that have been gaining popularity in recent years. Hyperspectral microscopy combines spectroscopy with optical microscopy to obtain both spatial and spectral information simultaneously, while remote sensing via ratiometric luminescence thermometry using trivalent lanthanide ions, Ln(III), allows for temperature determination based on well-established physical principles. The relevance of hyperspectral imaging in biomedical applications has become increasingly apparent, especially in the identification of pathogens and cancerous cells, monitoring bioimaging agents, and the cellular uptake of nanoparticles. On the other hand, remote sensing via ratiometric luminescence thermometry is a promising technique that has numerous applications and has gained popularity in recent years. However, most currently available Ln(III)-based luminescent thermometers require calibration using a reference thermal probe (secondary thermometers) and require frequent recalibration, which can be impractical, particularly when used in different media. Due to the challenges of calibration, primary thermometers based on well-established physical principles are the only way to ensure accuracy. While primary luminescent thermometers are rare, combining ratiometric data from the excitation spectrum of Ln(III) with the emission spectrum can lead to thermometers that do not require calibration (primary-S). The accuracy of these thermometers can be unparalleled, with an accuracy of 0.2% achieved in the physiological range. In the case of Yb3+/Er3+-codoped Gd2O3 nanoparticles, hyperspectral imaging was utilized to identify their location and track their movement within the cell structure. By analyzing 2D optical images acquired in different planes along with the cell culture depth, the internalization of the particles by MNT1 cells and their 3D localization in a fixed configuration were determined. The emission signature of the nanoparticles also enabled the determination of the intracellular temperature. Although the techniques are different, both hyperspectral microscopy and remote sensing via ratiometric luminescence thermometry have demonstrated their potential in biomedical applications and could pave the way for further advancements in the field.

MEMBROS DA BANCA:
Externo ao Programa - 1129726 - CID BARTOLOMEU DE ARAUJO - nullExterno à Instituição - HERMI FELINTO DE BRITO
Externo à Instituição - LEONIS LOURENÇO DA LUZ - UFPE
Presidente - 1134371 - RICARDO LUIZ LONGO