Bacterial cellulose films from kombucha with seriguela pulp and waste: characterization and application
kombucha; bacteria cellulose; seriguela; biofilm.
Kombucha is a fermented Asian drink consumed all over the world by people seeking a healthier lifestyle. A by-product of kombucha is the formation of bacterial cellulose, which develops on the surface of the drink and it can be used as a raw material due its excellent properties. In this study, bacterial cellulose films were obtained from the production of kombucha in different cultivation media, the incorporation of seriguela pulp and its extract into BC was evaluated and films were formulated with BC, glycerol and seriguela extract. The highest yield of CB was obtained from the 100% seriguela pulp medium, with 9,876g/L, while the lowest yield was from the 100% green tea medium with 5,330 g/L. There was a decrease in crystallinity in the films made with seriguela pulp, which ranged from 35% to 43%, compared to the membranes made without seriguela pulp and extract (51.18%). In the FTIR, the characteristic bands were similar to those of bacterial cellulose, with the intensity of the signals higher in the films produced with seriguela pulp. The CB membranes from kombuchas showed antimicrobial activity against E.coli and S. aureus, but the films obtained from the seriguela pulp and its extract were unable to prevent the microbial growth of E.coli, with only a halo of inhibition for S.aureus. The membranes incorporated with seriguela pulp had greater thermal stability and showed 3 stages of thermal degradation, with a mass loss of 70%. The films made with CB, seriguela pulp (20% and 30%) and glycerol (2%) showed antioxidant activity, with the highest value for the film with 20% seriguela (99.15%). There was a reduction in opacity, light transmission and crystallinity in the films incorporated with seriguela pulp at all concentrations. The films showed antimicrobial activity against S. aureus. In the infrared analysis (FTIR), the bands were compatible with those of bacterial cellulose. The films produced have suitable characteristics for use as antimicrobial and antioxidant packaging.