Banca de DEFESA: LUCAS COELHO BERNARDO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LUCAS COELHO BERNARDO
DATE: 25/01/2022
TIME: 14:00
LOCAL: Videoconferência
TITLE:

INTERAÇÕES BIOMOLECULARES DO VÍRUS ZIKA EM PROCESSOS PATOLÓGICOS DE MORTE CELULAR

KEY WORDS:

Replication cycle; Hydrogen bonds; Macroautophagy; Structural proteins; Viral RNA.

PAGES: 182
BIG AREA: Ciências Biológicas
AREA: Imunologia
SUMMARY:

Glycoproteins E e M of the Zika virus (ZIKV) participate in infectivity of the host cell through anchoring to cell surface receptors and fusion among membranes. However, little is known about the interaction of these ZIKV proteins with the receptors used by this virus for binding and/or entry in human cells. TNF type 1 (TNFR1), TNF type 2 (TNFR2) and Fas receptors are main members in family of transmembrane proteins due to the complete modulation of the extrinsic pathway of apoptosis. In this work, objective was to analyze the biomolecular interactions in silico and in vitro of the virus-cell relationship, evaluating the influence of ZIKV in cell death pathological processes. The silico analyzes were based on protein structures present in the RSCB Protein Data Bank (PDB) database. Protein-protein predictions were performed by the ClusPro server and molecular dynamics were performed using the GROMACS software. For in vitro analyzes, VERO cell lines and human neuroblastoma (SH-SY5Y) were used for viral expansion of the Pernambuco ZIKV strain and cell death assays. SH-SY5Y cells were subjected to treatments with a stimulant/inhibitor of the autophagic process (Rapamycin®, Chloroquine® or SBI-0206965®) and incubated for 0, 24 or 48 hours. From the supernatant, the ZIKV RNA and the cell quantity divided in two were obtained, to be performed as cell death analyzes for flow cytometry analyzes. Quantitative comparison was performed using two-way ANOVA. Here, the ZIKV protein M includes strong binding affinity and formation of interactions with the Fas and TNFR2 receptors. In addition, only the group with Chloroquine® exhibited a significant increase in viral quantity in relation to time and autophagy. Our work suggests that the ZIKV M protein may be an attachment factor for anchoring on Fas and TNFR2, as well as, an attenuated autophagy in the ZIKV infection leads to neuronal cell death.

BANKING MEMBERS:
Interna - 1675206 - DANYELLY BRUNESKA GONDIM MARTINS
Externa à Instituição - ELISA DE ALMEIDA NEVES AZEVEDO - Fiocruz - PE
Presidente - 1701590 - LUCAS ANDRE CAVALCANTI BRANDAO