Banca de DEFESA: ÉDIPO LUCAS SOARES BARBOSA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ÉDIPO LUCAS SOARES BARBOSA
DATE: 09/11/2022
TIME: 14:00
LOCAL: goole meet
TITLE:

PHYTOCHEMICAL PROFILE, TOXICITY ASSESSMENT AND ANTINOCICEPTIVE ACTIVITY OF LEAVES EXTRACT OF Psidium guineense Sw.

KEY WORDS:

Antinociceptive activity; Araça; Acute toxicity; Medicinal plants; Opioid system.

PAGES: 70
BIG AREA: Ciências Biológicas
AREA: Farmacologia
SUMMARY:

Species of the genus Psidium have been reported as source of antimicrobial, anti-inflammatory, antinociceptive, cytotoxic, antipyretic and antioxidant activities. Psidium guineense (Myrtaceae), known as “araçá”, is cultivated in North, Central and South American Countries. The aim of this work was to characterize the methanolic extract of P. guineense leaves (EMPg) for its phytochemical composition, in vivo toxicity and antinociceptive potential. Dried leaves were ground and the powder was analyzed for secondary metabolite content by thin layer chromatography (TLC). Then, a factorial design (2³) was carried out to determine the most efficient method of extraction, using as independent variables the extractive method (maceration or turbolysis), mass of leaves (5 or 10 g) and solvent proportion (50% or 100%, v/v methanol). The preparation (EMPg) with the highest extraction efficiency (ratio between phenol content and dry residue) was analyzed by high performance chromatography with diode array detector (HPLC-DAD). Next, EMPg was investigated for acute toxicity using Swiss albino mice and for antinociceptive activity using the acetic acid-induced writhing test (0.1%), the formalin test (2.5%) and the tail immersion test. The involvement of opioid receptor in the antinociceptive mechanism of EMPg was evaluated by intraperitoneal pretreatment of animals with naloxone. The analysis of P. guianeense leaf powder by TLC revealed the presence of flavonoids, cinnamic derivatives, saponins, hydrolysable tannins, sugars and terpenes/steroids. Higher extraction efficiency was recorded for EMPg, obtained using 5 g of leaves by maceration in 50% methanol. HPLC-DAD of EMPg indicated the presence of monomers of hydrolysable tannins, gallic acid, ellagic acid and flavonoids. Treatment with EMPg resulted in a small increase of weight, and water and feed consumption by the animals, as well as in slight changes in hematological and biochemical parameters, without clinical relevance. EMPg exhibited analgesic activity, reducing the number of abdominal writhes and paw licking time in both phases of the formalin test. Additionally, a progressive increase in the latency time in the tail immersion test was recorded. Pretreatment with naloxone reduced the antinociceptive effect of EMPg, suggesting the involvement of the opioid system in the antinociceptive action of the extract. In conclusion, the leaves of P. guianeense are sources of metabolites with pharmacological potential for presenting antinociceptive action in an in vivo model, without causing acute toxicity.

COMMITTEE MEMBERS:
Presidente - ***.833.854-** - EMMANUEL VIANA PONTUAL - UFPE
Externa ao Programa - 1328775 - MAGDA RHAYANNY ASSUNCAO FERREIRA - UFPEInterna - 1133984 - PATRICIA MARIA GUEDES PAIVA
Interno - 1960817 - THIAGO HENRIQUE NAPOLEAO