Mixture Design of Inocula for Enhanced Biomethane Production from Co-digestion of Fruit and Vegetable Waste
Anaerobic digestion, Cattle rumen content, wastewater biosolids, fruit and vegetable waste, mixture design, effect plots
Anaerobic digestion (AD) is one of the most widely used bioconversion technologies for the production of renewable energy from organic waste due to its ability to convert organic carbon into CH4 and CO2. Therefore, the aim of the present work was to quantitatively investigate the influence of inocula mixing on the digestion of fruit and vegetable waste (FVW) for enhanced biomethane production. The inocula mixture consisted of digestate from an anaerobic digester inoculated with anaerobic sludge from an AD plant, liquid biofertilizer, cattle rumen content and anaerobic sludge. The results show that digestion of FVW with an inoculum ratio of 25 %:75 % liquid biofertilizer and rumen fluid respectively, is an optimum for enhancement of biomethane production (453.12 Nml/gSV), while digestion with a ratio of 25 %:75 % liquid biofertilizer and digestate from a AD plant resulted in the lowest biomethane production (256.7 Nml/gSV). The overall results indicate that the presence of rumen fluid in the inocula mixture had a significant effect, a synergy between biofertilizer and rumen, on increasing biomethane production and anaerobic biodegradability of FVW and thus may contribute to higher efficiency in AD. While the presence of digestate in the inocula mixture had a negative effect, an antagonism between biofertilizer and digestate.