STRATEGIES FOR BIOREMEDIATION OF NITRO AND CHLOROAROMATIC COMPOUNDS FROM A CONTAMINATED SITE IN BAHIA
Nitro and chloroaromatics. Bioremediation. 3-nitrochlorobenzene. Native microorganisms. Aerobic biodegradability.
Chlorinated anilines and chlorinated nitrobenzenes are intermediates in the production of dyes, pharmaceuticals, perfumes, pesticides, herbicides, cosmetics and explosives. There are reports of environmental contamination by these compounds at an old herbicide manufacturing site in the state of Bahia. The remediation of these pollutants is imperative for environmental protection, as they are toxic and mutagenic. This work presents strategies for bioremediation of nitro and chloroaromatics in this contaminated site. First, the ability of native microorganisms to aerobically degrade the main contaminants present in groundwater and identify them was verified. Microorganisms that degrade 2,3- and 3,4-dichloroaniline (DCA), 2,3- and 3,4-dichloronitrobenzene (DCNB) and 3-nitrochlorobenzene (CNB) were isolated and their genome will be sequenced. There are no reports in the literature of microorganisms capable of degrading 3-CNB. Afterwards, respirometry tests were performed to investigate the presence of minerals or dissolved organic compounds in the contaminated site's groundwater, which are not identified as worrying contaminants, but exert biochemical oxygen demand to be considered in the O2 supply project of a pilot biosparging system developed by a consulting company. From this same system, the density of specific microbial groups was estimated using the Most Probable Number (MPN) technique. Then, the ability of laboratory-grown microorganisms to degrade target contaminants when present in soil was verified, including inhibition assessment of microbial activity. Finally, we want to obtain parameters such as pH and moisture content and identify the presence of native microorganisms that degrade the contaminants present in the soil to support the development of a pilot biopiles project for soil bioremediation..