Banca de DEFESA: GABRIEL VICTOR DE LIMA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : GABRIEL VICTOR DE LIMA
DATE: 31/07/2023
TIME: 13:30
LOCAL: Pós-Graduação Engenharia Civil
TITLE:

Taxonomic Evaluation of Different Microbial Cultures Adapted to Anaerobic Degradation of Aniline

KEY WORDS:

aniline; biodegradation; taxonomy; methanosaeta; aminicenantes; geobacter.

PAGES: 53
BIG AREA: Engenharias
AREA: Engenharia Civil
SUMMARY:

Aniline is a molecule composed of a benzoic group linked to an amino group. Although aniline is an important raw material to produce pharmaceuticals, dyes, pigments, and other economically valuable products, its toxicity and carcinogenic activity to animals and plants make it a potential environmental contaminant. Aniline can act both as a precursor molecule and as a byproduct released during the aerobic or anaerobic degradation of various chemical compounds. The disposal of waste containing this substance can pose a risk to the environment and public health. While anaerobic degradation of aniline is possible, and some possible steps in the degradation pathway have been elucidated, there are still gaps that require investigation, especially regarding the use of mixed cultures. This study evaluated the taxonomic composition of three distinct mixed cultures adapted to anaerobic degradation of aniline using 16S rRNA sequencing. The degradation kinetics of aniline and the formation of byproducts were analyzed using high-performance liquid chromatography. The degradation kinetics of aniline increased with successive feedings, reaching 0.99 μM/day of aniline. Different concentrations of the byproducts benzoic acid and 4-aminobenzoic acid were recorded. It was possible to observe that despite the different origins of the microbiotas, the microbial core of the microcosms with sediment from a river, a textile reactor, and a petrochemical source were selected in a similar manner. The common groups that stood out the most were Methanosaeta, Aminicenantes, and Geobacter.

COMMITTEE MEMBERS:
Externo à Instituição - JOSE ROBERTO SANTO DE CARVALHO - UFPE
Externa à Instituição - MARCIA HELENA RISSATO ZAMARIOLLI DAMIANOVIC - USP
Presidente - 2304950 - WANDERLI ROGERIO MOREIRA LEITE