Banca de DEFESA: OHANNA MARIA MENEZES MADEIRO DA COSTA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : OHANNA MARIA MENEZES MADEIRO DA COSTA
DATE: 13/08/2021
TIME: 14:00
LOCAL: Plataforma online
TITLE:

Production of Nanoliposomes with High Potential for Nanomedicine by Hydrodynamic Focusing
Microfluid Device

KEY WORDS:

Anionic Nanoliposomes. Stealth Nanoliposomes. Microfluidics. Nanomedicine.

PAGES: 105
BIG AREA: Outra
AREA: Multidisciplinar
SUMMARY:

Drug Delivery Systems (DDS) is a promising area of nanomedicine that has been actively developed,
aiming to maximize the therapeutic effects of drugs. The efficiency in the DDS performance depends on
the system used and its characteristics. In this sense, liposomes are very attractive systems, as they present
naturally several desirable characteristics, such as biocompatibility, biodegradability, stability in biological
fluids, different chemical environments in their structure and they are not immunogenic. Among the types
of liposomes, anionic liposomes (AL) have received significant attention in recent years due to the potential
for nanomedicine and for having some formulations already launched for clinical development. However,
their application present two major challenges: laborious methods of production and rapid degradation and
elimination from blood by the immune system. This work aimed to overcome these challenges through
microfluidics – which is fluid engineering on a microscale – and the development of stealth liposomes to
the immune system. We used a single-stage microfluidic hydrodynamic focusing (MHF) process and
studied the variation of some parameters, including the flow rate ratio (FRR), to optimize the production of
nanoliposomes with commercial formulation. We optimized the AL production process composed by the
commercial formulation DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine) and DMPG (1,2
dimyristoyl-sn-glycero-3-phospho-(1'rac-glycerol) (sodium salt)) and its physicochemical and
morphological properties, obtaining unilamellar, small (53.7 ± 1.4 nm) and near-monodispersed (0.080 ±
0.009) AL relative to commercial formulations launched for clinical development. Also, when compared
with conventional methods of production, no post-processing step was necessary to obtain AL with the
desired characteristics. Our work also demonstrated that using this technique, it is possible to upgrade the
AL formulation to its stealth form (SAL), again using a single step process, obtaining a unilamellar and
monodispersed system (0.060 ± 0.004), whereas, by conventional methods, SALs have high polydispersity
(e. g. 0.309). Thus, our study demonstrated that the microfluidic technique has great potential for producing
of AL and SAL with controlled sizes and reproducible characteristics in a single step, revealing essential
implications for this technology's potential usefulness in the chemical and pharmaceutical industry, and the
features of the nanomaterials applied in nanomedicine.

BANKING MEMBERS:
Externa à Instituição - SAYONARA ANDRADE ELIZIÁRIO
Externo à Instituição - LUIZ GONZAGA DE FRANCA LOPES
Interna - 3199308 - PATRICIA MARIA ALBUQUERQUE DE FARIAS
Presidente - 1354207 - SEVERINO ALVES JUNIOR
Interna - 1283002 - YEDA MEDEIROS BASTOS DE ALMEIDA