Banca de DEFESA: DÁRIO CÉSAR DE OLIVEIRA CONCEIÇÃO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : DÁRIO CÉSAR DE OLIVEIRA CONCEIÇÃO
DATE: 13/11/2023
TIME: 09:00
LOCAL: Programa de Pós-Graduação em Ciência de Materiais
TITLE:

SÍNTESE AND CHARACTERIZATION OF MESOPOROUS NANOSPHERES OF MAGNETITE AND COBALT FERRITE FOR BIOMEDICAL APPLICATIONS

KEY WORDS:

Nanospheres, Cobalt Ferrite; Magnetite; Biomedical Applications.

PAGES: 95
BIG AREA: Outra
AREA: Multidisciplinar
SUMMARY:

Magnetic nanoparticles have garnered significant interest in the biomedical field due to their unique properties, such as their size being comparable to cellular structures and their ability to respond to magnetization gradients. In the medical context, these applications encompass a wide range of functionalities, ranging from their use as contrast agents to serving as vehicles for drug delivery. The primary objective of this study revolves around the synthesis and characterization of spherical and mesoporous nanoparticles of Cobalt Ferrite (CoFe2O4) and Magnetite (Fe3O4) for biomedical applications, including their role as drug delivery systems, biological sensors, and in magnetic hyperthermia therapy. The synthesis of the particles was conducted using the solvothermal method assisted by Cetyltrimethylammonium Bromide (CTAB), which acted as a templating agent, creating mesopores within the nanoparticles. The morphology of the particles was characterized using Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM), revealing the formation of mesoporous spheres with an average size of 132,49 ± 21 nm for the Cobalt Ferrite samples and 75,90 ± 12,55 nm for the Magnetite samples. Chemical composition and crystal structure characterization were performed using X-ray Diffraction, confirming the formation of an inverse spinel structure, characteristic of both Cobalt Ferrite and Magnetite materials. Furthermore, Fourier Transform Infrared Spectroscopy (FTIR) analysis revealed a peak around 580 cm-1, corresponding to the metal-oxygen bond in the Ferrite and Magnetite structures, which was further confirmed by diffraction. Drug absorption assays, based on absorbance measurements, allowed for the assessment of nanoparticle drug storage capacity. It was observed that Cobalt Ferrite nano-spheres were capable of storing 46,85% of the drug in solution, while Magnetite nanoparticles stored 36,91%. Notably, regarding drug release, significant efficiency was observed at a temperature of 43°C, with 62,68% drug release for Cobalt Ferrite particles and 88,12% for Magnetite particles. In hyperthermia assays, Cobalt Ferrite particles demonstrated efficacy as hyperthermia-inducing agents, exhibiting a temperature variation of 9,2°C. The magnetic profile of the particles was confirmed using a technique quantifying saturation magnetization values. Cobalt Ferrite particles showed potential as components of a biosensor system, evidenced by their positive response when antibodies were immobilized on them. The assay involving the conjugation of Cobalt nanoparticles with immunoglobulins and their application in lateral flow tests holds promising prospects for the development of a lateral flow biosensor.

COMMITTEE MEMBERS:
Externa à Instituição - DULCIENE KARLA DE ANDRADE SILVA - UFRPE
Externo à Instituição - ALVARO ANTONIO OCHOA VILLA - IFPE
Presidente - 2457389 - EDUARDO PADRON HERNANDEZ
Externo à Instituição - JOSE ANGELO PEIXOTO DA COSTA - IFPE
Externo à Instituição - YUSET GUERRA DAVILA - UFPI