Banca de DEFESA: ELTON MARLON DE ARAUJO LIMA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : ELTON MARLON DE ARAUJO LIMA
DATE: 22/09/2023
TIME: 09:00
LOCAL: Programa de Pós-Graduação em Ciência de Materiais
TITLE:

DEVELOPMENT OF A MAGNETIC NANOCOMPOSITE BASED ON IRON OXIDE AND CONDUCTIVE POLYMER (γ-Fe2O3-POLYTHIOPHENE) AS A PROPOSED THERAPEUTIC PLATFORM AGAINST TEGUMENTARY LEISHMANIASIS

KEY WORDS:

Magnetic nanocomposite; tegumentary leishmaniasis; nanotechnology.

PAGES: 165
BIG AREA: Outra
AREA: Multidisciplinar
SUMMARY:

In this work, we propose a new nanobiotechnological therapeutic platform, based on the use of a nanocomposite of magnetic iron oxide (γ-Fe2O3) and a conductive polymer (Polythiophene - PTh), developed to overcome the limits of the conventional treatment of cutaneous leishmaniasis. After being synthesized via the Fenton reaction, the hybrid magnetic nanocomposite γ-Fe2O3/PTh was characterized by UV-Vis, FTIR, SEM-EDS, and magnetization and particle size measurement techniques. The effective coating of PTh on the particles of the γ-Fe2O3/PTh nanocomposite was confirmed by spectroscopic measurements, which showed an average size of 41 nm and superparamagnetic behavior, with a magnetization saturation of 15.7 emu/g at room temperature. Then, the cytocompatibility profile of the nanocomposite with mammalian cells and human erythrocytes was investigated, as well as its antiLeishmania potential against two species that cause tegumentary leishmaniasis (L. amazonensis and L. braziliensis) in their two stages of development (promastigotes and amastigote). When we implemented the cytocompatibility studies, we verified that the γ-Fe2O3/PTh nanocomposite did not reduce the viability of the cells tested (peritoneal macrophages, fibroblasts, and vero), having shown a low capacity to cause rupture in human red blood cells of different phenotypes, which indicates a good hemocompatibility. Overall, the in vitro cytotoxicity studies of the γ-Fe2O3/PTh nanocomposite showed it to have a promising cytocompatibility profile, opening the possibility of its use in therapeutic formulations. Finally, it was also possible to show that this nanocomposite significantly inhibited the growth of promastigotes of L. amazonensis (with IC50 = 117.63 μg/mL). For the intracellular amastigote cell form, the one most pathogenic for the human host, the γ-Fe2O3/PTh nanocomposite showed a significant inhibition effect, with an IC50 of 27.1 μg/mL (L. amazonensis) and 16.6 μg /ml (L. braziliensis). It was also verified that the γ-Fe2O3/PTh nanocomposite can cause a more intense depolarization of the mitochondrial membrane on the promastigote forms of L. amazonensis. Our results indicate that the γ-Fe2O3/PTh nanocomposite emerges as a promising material for advances in the treatment of tegumentary leishmaniasis through the proposal of a nanobiotechnological product that would offer good efficacy against different species of parasites without causing damage to the host cell (macrophage).

COMMITTEE MEMBERS:
Externo à Instituição - JOSMARY RODRIGUES SILVA - UFMT
Externo à Instituição - RICARDO BENTES DE AZEVEDO - UnB
Interna - 2199290 - BEATE SAEGESSER SANTOS
Presidente - 1130294 - CELSO PINTO DE MELO
Externo à Instituição - FABIO ROCHA FORMIGA - UPE