Banca de DEFESA: LAISA WANESSA SANTOS LIMA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LAISA WANESSA SANTOS LIMA
DATE: 16/11/2022
TIME: 10:00
LOCAL: Pós-Graduação em Saúde Translacional
TITLE:

AVALIAÇÃO DO EFEITO DA FRAÇÃO POLAR DO EXTRATO DE PARTES AÉREAS DE Parkinsonia aculeata L. SOBRE COMPONENTES DO SISTEMA RENINA-ANGIOTENSINA (SRA) E EM MARCADORES DE ESTRESSE OXIDATIVO NO TECIDO CARDÍACO DE RATOS COM SÍNDROME METABÓLICA

KEY WORDS:

Parkinsonia Aculeata. Diabetes Mellitus. Hypertension. Obesity. Dyslipidemias.

PAGES: 30
BIG AREA: Ciências da Saúde
AREA: Fisioterapia e Terapia Ocupacional
SUMMARY:

Changes in eating habits and a sedentary lifestyle over the last few years have influenced the increase in obesity, considered a worldwide epidemic. The increase in energy supply through a diet rich in simple carbohydrates, lipids and low in fiber, linked to the reduction of physical activity, called contemporary western lifestyle, triggers metabolic disorders such as dyslipidemia, type 2 diabetes mellitus, cardiovascular diseases, among others. In this context, the purpose of the study was to analyze the effect of the polar fraction of the extract of aerial parts of Parkinsonia aculeata L. (PfrEHPA) on RAS components and on oxidative stress markers in the cardiac tissue of male Wistar rats after weaning ( 21 days) initially divided into two groups that received a standard diet for rodents (Presence ®) or a “Westernized” diet (OD) for 150 days. Then, these animals were divided into 5 subgroups which were treated orally for 30 consecutive days with PfrEHPA (70 or 140 mg/kg/day), metformin (500 mg/kg/day) or distilled water (10 mL/day). kg/day). During the 150 days of diet consumption, the weekly food consumption, weight evolution, body dimensions and lipid profile of the animals were recorded. These same parameters were determined again after the treatment described above. At the end of the experiment (180 days) blood samples were collected to determine the hematological and biochemical profile. Subsequently, the animals were euthanized under anesthesia and decapitation and then the organs were weighed and stored, part in formalin (10%) for later histological and immunohistochemical analysis, and part stored in a freezer with a temperature maintained at -80ºC, which was used for the analysis of oxidative extraction. Our results showed that animals submitted to the Westernized Diet showed signs of metabolic syndrome (increased waist circumference, hyperlipidemia, hyperglycemia). The change in the lipid profile of the DO group was evidenced by a significant (p<0.05) increase in serum levels of total cholesterol (91 ± 1.57), LDL (37.2 ± 2.05) and triglycerides (135, 67 ± 2.09) in relation to the PD group (64.67 ± 1.16 ; 23.67 ± 1.88 and 75 ± 4.01, respectively). These animals also showed a significant (p<0.05) increase in fasting glucose (mg/dl) compared to animals that ingested PD. The treatment with PfrEHPA (140 mg/kg), even with the continued consumption of the westernized diet by the animals (150th – 180th days), promoted a significant reduction in the levels of total cholesterol (62.33±0.80), LDL (21 .70±0.63) and triglycerides (68.16±0.79) in relation to the untreated OD group (92±0.73; 37.77±1.45 and 141.17±1.14, respectively). Additionally, treatment with PfrEHPA (140 mg/kg) also reduced serum glucose levels, systolic blood pressure and levels of oxidative stress in the cardiac tissue of these animals.

COMMITTEE MEMBERS:
Presidente - 1130527 - IVONE ANTONIA DE SOUZA
Externa ao Programa - 1132302 - GLORIA ISOLINA BOENTE PINTO DUARTE - UFPEExterna à Instituição - SINARA MÔNICA VITALINO DE ALMEIDA - UPE