Banca de DEFESA: LARISSA CAROLINE DE ALMEIDA SOUSA LIMA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LARISSA CAROLINE DE ALMEIDA SOUSA LIMA
DATE: 24/08/2022
TIME: 15:30
LOCAL: Pós-Graduação em Saúde Translacional
TITLE:

EVALUATION OF THE EFFECT OF A BIOPRODUCT OBTAINED FROM Parkinsonia aculeata ON COMPONENTS OF THE RENIN-ANGIOTENSIN SYSTEM (RAS) IN THE RENAL TISSUE OF RATS WITH EVIDENCE OF METABOLIC SYNDROME

KEY WORDS:

Obesity, Parkinsonia aculeata, metabolic syndrome, westernized diet

PAGES: 84
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

Obesity is recognized as a global public health problem, associated with the development of several non-communicable chronic diseases that can cause disorders and alterations such as those observed in metabolic syndrome (SM). Thus, the objective was to evaluate the effect of the polar fraction obtained from the aerial parts of Parkinsonia aculeata (PfrHEPA), the expression of the AT1 receptor for angiotensin II in the renal tissue in an experimental model of SM. For SM induction, 24 male Wistar rats were fed a Western diet (DO) for 150 days, divided into 4 groups (n=6) and the control group (n=6) was fed a standard diet (SD). During the 150 days, the animals were monitored weekly for weight gain, and at the end of this period, the systemic blood pressure (SBP) was evaluated and blood was collected to evaluate the lipid, glycemic and hematological profile, to verify the establishment of SM in these animals. The MS groups maintained their DO and were treated for 30 consecutive days with PfrHEPA 70mg/kg, PfrHEPA 140mg/kg), metformin 500mg/kg or vehicle 10mL/kg, orally. At the end of treatment, PAS was determined and blood was collected for biochemical and hematological evaluation. Subsequently, the animals were euthanized under anesthetic depth to collect the renal tissue in order to analyze the expression of the AT1 receptor for angiotensin II by means of immunohistochemistry. It was evidenced that all animals submitted to DO developed SM, presenting obesity, dyslipidemia, hyperglycemia and arterial hypertension. After treatment, the animals that received PfrHEPA (70mg/kg or 140mg/kg) showed a significant reduction in serum levels of total cholesterol (22% and 32%); triglycerides (44% and 51%) and blood glucose (27% and 43%) respectively; in relation to blood pressure levels, treatments at the same doses reduced by 22% and 24%, respectively. This result is in line with the immunohistochemical findings, where there was a reduction in the expression of the AT1 type receptor of angiotensin II in the glomerulus and renal tubule of the treated animals. In the histopathological analysis of the renal tissue, no morphostructural changes were observed. Thus, it is concluded that PfrHEPA, regardless of the doses tested, was able to reduce the expression of the AT1 receptor and modulate blood pressure in Wistar rats with SM.

BANKING MEMBERS:
Externa ao Programa - 1132498 - BELMIRA LARA DA SILVEIRA ANDRADE DA COSTA
Externo à Instituição - ERYVELTON DE SOUZA FRANCO
Presidente - 2284980 - GUSTAVO PINA GODOY