Banca de DEFESA: VERA KAISSA SOUZA SANTOS BACELAR

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : VERA KAISSA SOUZA SANTOS BACELAR
DATE: 30/01/2024
TIME: 09:00
LOCAL: Pós-Graduação em Saúde Translacional
TITLE:

HISTOPATHOLOGICAL EVALUATION AND DETERMINATION OF EXPRESSION OF SURFACTANT PROTEIN TYPE-B (SP-B) IN THE LUNGS OF SENTIC RATS TREATED WITH CEFTRIAXONE AND ANTIOXIDANTS.

KEY WORDS:

Surfactant Protein B, SP-B, Acute Lung Injury, Sepsis.

PAGES: 60
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Introduction: Sepsis is defined as a potentially fatal organ dysfunction caused by an insufficient response in controlling the infectious agents involved. Due to the increasing number of cases, high mortality rate and high cost of treating sepsis, it has become a public health problem. Sepsis is among the main diseases involved in the onset of acute lung injury (ALI), since the lung is one of the organs most commonly affected. Objective: Histopathological evaluation and determination of surfactant protein type-b (sp-b) expression in the lungs of septic rats treated with ceftriaxone and antioxidants. Methodology: A total of 70 male Wistar rats (200-350g) were used to compose seven experimental groups (n=10/group). Of these, 40 were submitted to induction of sepsis by Cecal Ligation and Puncture (CLP) and subdivided into the treated: I) Distilled water (5 ml/kg; i.p.) = CLP+AD; II) ceftriaxone (30 mg/kg; i.p.) = CLP+ATB; III) antioxidants (A. Ascorbic acid 50mg/kg, NAC 10mg/kg and Alpha tocopherol 20mg/kg; i.p) = CLP+ATO; IV) ceftrianone (30 mg/kg; i.p.) and antioxidants (Ascorbic A 50mg/kg, NAC 10mg/kg and alpha tocopherol 20mg/kg; i.p) = CLP+ATO+ATB. The SHAM group (control) was composed of three groups (n=10/group), which underwent the same surgical procedure without inducing sepsis and were treated in the same way as CLP: I) SHAM+DA; II) SHAM+ATB; III) SHAM+ATB+ATO. After 24 hours of the procedure, the animals received the treatment for five consecutive days. During this period, the animals were observed for functional/physiological characteristics and, at the end, they were anesthetized for blood collection, determination of the hematological profile and then euthanized by anesthetic deepening to collect the left lower lung lobe for histomorphometric evaluation (quantification of neutrophils, macrophages , lymphocytes, type II pneumocytes, alveoli and 'determination of alveolar area and thickness of alveolar septa) and determination of SP-B protein expression by Western Blot. Results: The percentage of experimental mortality in the septic group was 21.42%, which made it possible to continue the groups with n=6. The survival rate of the CLP groups varied significantly (P<0.05) according to the type of treatment and observation time: CLP+AD (48h – 50.67%; 72h – 50.67%; 84h – 50, 67% and 96h – 50.67%); CLP+ACT (84h – 68% and 96h – 68%); CLP+ATB (48h - 34%; 72h – 34%; 84h – 34% and 96h – 18%) when compared to the SHAM+AD group at the same times. As for the physiological characteristics, the septic animals treated with ATO or ATB+ATO showed a significant improvement (P<0.05) in the condition of mobility, lethargy and diarrhea compared to the CLP+AD group. In the hematological evaluation, the platelet count (103/dL) was significantly reduced (P<0.05) in the CLP+ATB group (331.33±131) and this decrease was not verified in the CLP+ATB groups (627± 25.32) and CLP+ATB+ATO (437.33± 97.88) when compared to SHAM+AD (429± 69). As for histomorphometry, it was verified that the infiltration of inflammatory cells in the lung tissue of animals treated with ATO or ATB+ATO did not present a significant difference compared to the SHAM+AD group, on the other hand, animals treated with CLP+AD or ATB showed a significant increase (P<0.05) of inflammatory cells compared to that observed in SHAM+AD. Regarding the number of type II pneumocytes, the groups treated with ATO or ATB+ATO showed no significant difference compared to SHAM+AD. However, the one treated with ATB alone significantly reduced the amount of type II pneumocytes in this group compared to all others. The alveolar quantity in the CLP animals treated with ATO alone were similar to those verified in the SHAM+AD and CLP+ATB+ATO groups. The septic animals treated with ATB had a greater number of alveoli, but with a smaller alveolar area compared to the other groups. In turn, the alveolar area in the septic groups treated with ATB; ATO or ATB+ATO exhibit significantly smaller area compared to SHAM+AD. With regard to the thickness of the alveolar septa of animals treated with ATO or ATB+ATO, these were similar to SHAM+AD. This fact was not verified in septic animals treated with ATB alone, which presented septal thickness greater than those observed in animals treated with ATO or ATO+ATB. Conclusion: Given the above, antioxidants (vitamin C, vitamin E and N-acetylcysteine) when administered intraperitoneally in an animal model of sepsis, associated or not with an antibiotic, seem to promote the conservation of the histological structures present in the pulmonary matrix even when subjected to injuries resulting from this infectious disease, and increases the survival of animals that received these treatments.

COMMITTEE MEMBERS:
Externo à Instituição - ANDRE MARTINS GALVAO - UNICAP
Interno - 2929021 - ERYVELTON DE SOUZA FRANCO
Interno - 3617065 - MARIO RIBEIRO DE MELO JUNIOR