Banca de QUALIFICAÇÃO: DENISE MAIARA DA SILVA CARNEIRO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : DENISE MAIARA DA SILVA CARNEIRO
DATE: 24/08/2023
LOCAL: PÓS GRADUAÇÃO EM SAÚDE TRANSLACIONAL
TITLE:

MONITORING OF CHILD AND TEENS’ ADHESION TO ALLERGIC RHINITIS PROPHYLAXIS BY NMR-BASED METABONOMICS ASSAYS

KEY WORDS:

metabolic profile. predictive models. disease management. Asthma. self-report. Rhinitis.

PAGES: 52
BIG AREA: Ciências da Saúde
AREA: Enfermagem
SUMMARY:

Allergic rhinitis is a chronic condition prevalent in children and adolescents, characterized by inflammation of the nasal mucosa in response to allergens. Adherence to treatment is essential for controlling symptoms and improving the quality of life of patients. The present study aimed to develop a metabonomic model based on 1H NMR spectroscopy to assess adherence to treatment in children and adolescents with allergic rhinitis. For the study, 39 patients with allergic rhinitis were recruited, divided into two groups based on adherence to treatment (good and poor adherence), considering the report of the patient or guardian. Serum samples were collected from participants and analyzed using the 1H Nuclear Magnetic spectroscopy technique to obtain metabonomic profiles. To calculate the models, Principal Components Analysis (PCA) and Hierarchical Cluster Analysis (HCA) were initially used. In the classificatory analysis, the Discriminating Orthogonal Least Squares Analysis (OPLS-DA) was used. The most relevant metabolites for the construction of the OPLS-DA model were identified through the importance of the variable in the projection (VIP). Regarding the study participants, the average age was nine years, with a predominance of males. Most participants were diagnosed with moderate/severe allergic rhinitis and also had concomitant asthma. Of the participants, more than half (n=24) reported adherence to treatment. The model using PCA of the metabonomic data showed no group distinction. However, HCA identified two distinct groups, which did not align with adherence or non-adherence to treatment. After analyzing the medical records, it was found that the assessment of turbinate hypertrophy using rhinoscopy was related to the response trend observed in the groups identified by the HCA. Thus, the groups were named as Group RC, composed of 20 samples of patients who showed clinical response to pharmacological treatment, evidenced by involution or progression of turbinate hypertrophy, and Group IR, composed of 19 samples of patients indifferent to treatment, without improvement or clinical worsening of turbinate hypertrophy. In the constructed OPLS-DA model, fifteen relevant metabolites that contributed to the separation of groups were identified. In the clinical response group (CR), the identified metabolites were lactate, proline, ketoleucine, 2-oxoisocaproic acid, and aspartic acid. In the undetermined response group (IR), choline, leucine, 2-hydroxy-3-methylbutyric acid, VLDL/LDL, glucose, methylhistidine, alpha-glucose, guanidoacetic acid, methanol and glycerol were identified. These results suggest more significant metabolic changes in the IR group, including energy metabolism, amino acids and lipids. It is noteworthy that no metabonomic difference was observed between patients with good and poor adherence to treatment, based on self-reported adherence, highlighting the importance of combined approaches to assess adherence to treatment in patients with allergic rhinitis.

COMMITTEE MEMBERS:
Externa ao Programa - 2482412 - ANA CAROLINE CAVALCANTI DELA BIANCA MELO - nullExterno ao Programa - 1212348 - EDIL DE ALBUQUERQUE RODRIGUES FILHO - null