Banca de DEFESA: CAROLINA DIAS DA SILVA AMORIM

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CAROLINA DIAS DA SILVA AMORIM
DATE: 21/06/2023
TIME: 09:00
LOCAL: Hospital das Clínicas
TITLE:

EVALUATION OF CLINICAL IMPACT OF INTERLEUKIN 8 GENE EXPRESSION IN PATIENTS WITH FIBROMYALGIA.

KEY WORDS:

Fibromyalgia; interleukin 8; depression; fatigue; sleep; obesity.

PAGES: 73
BIG AREA: Ciências da Saúde
AREA: Medicina
SUMMARY:

Fibromyalgia (FM) is a musculoskeletal syndrome characterized by diffuse and chronic pain associated with other symptoms such as fatigue, sleep/cognition disorders, headache, depression, and anxiety, resulting from a change in pain processing. Some working has shown an increase in some interleukins (IL1, IL6, IL8, IL10, IL17 and TNF- α) in patients with FM when compared to controls, however, there is still no uniformity and consensus in the results. It is known that patients with FM have a higher frequency of obesity compared to the general population and recent studies have shown an increase in interleukin 8 (IL8) in patients with obesity as well as in FM. There is no study evaluating IL8 in FM and association with obesity and other clinical parameters. To verify the impact of IL8 gene expression on the clinical parameters of patients with FM and compare whether IL8 gene expression is increased in relation to the comparison group (CG). Cross-sectional, observational, descriptive and analytical study, carried out from August to December 2021, with the sample of cases consisting of patients diagnosed with FM (GFM) treated at the rheumatology service of the Hospital das Clínicas of the Federal University of Pernambuco (HC-UFPE). The CG was composed of patients' companions and hospital staff. Clinical and demographic data were collected in both groups, in addition to weight and height for calculating the Body Mass Index (BMI), application of questionnaires for: fatigue (Functional assessment of chronic illness therapy), depression (Beck depression inventory), sleep (numerical sleep scale) and impact of FM (Fibromyalgia Impact Questionnaire Revised) and peripheral blood collection for evaluation of IL8 gene expression analyzed through the difference between the target gene (IL8) and the endogenous control gene (B-actin). Study variables were: expression of IL8, sex, age, race, physical activity, duration of illness, occupational situation, comorbidities, use of medications, values of questionnaires for fatigue, sleep, depression and impact of fibromyalgia. Patients with FM did not show a higher frequency of IL8 gene expression compared to the CG, but among individuals who express IL8, the FM group had a higher gene expression. There was no association of IL8 with worse FIQ-R indices, sleep disturbance, BMI or fatigue, but there was an association with depression, where expressing IL8 showed worse indices in BDI (p= 0.002). Patients with FM do not have a higher frequency of IL8 expression compared to CG and patients with IL8 expression
have a greater association with moderate depression than patients who do not express IL8.

COMMITTEE MEMBERS:
Externa à Instituição - ALINE ROZALIN - UFPE
Externa à Instituição - NARA GUALBERTO CAVALCANTI - UFPE
Presidente - 2199064 - RICARDO OLIVEIRA DA SILVA