Banca de DEFESA: CYNTHYA MYLLENA MARTINS SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CYNTHYA MYLLENA MARTINS SILVA
DATE: 28/04/2023
TIME: 14:00
LOCAL: GOOGLE MEET
TITLE:
KEY WORDS:

Obesity; Serotonin Transporter; Chewing; Litter Size; Development plasticity.

PAGES: 59
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

The increase in body weight can be associated with changes in masticatory function. Under overweight and obese conditions, the control of energy balance is clearly deregulated and serotonergic modulation appears to contribute significantly to weight gain. This study aims to evaluate the effect of obesity induced by litter size reduction on somatic and masticatory parameters and serotonin transporter immunoreactivity (SERT) in masticatory trigeminal nuclei. The study, carried out with Wistar rats, was submitted and approved by the Ethics Committee on Animal Experimentation of the Federal University of Pernambuco (0026/2019). Rats were mated at a ratio of 2 females to 1 male and kept under standard vivarium conditions. Forty-eight male Wistar albino rats (Rattus Norvegicus) from progenitor rats from the vivarium colony of the Nutrition Department of the Federal University of Pernambuco were used. Seventy-two hours after birth, the animals were randomly distributed into two groups: Control (C; 9 pups per nursing mother; n=24) or overfed (SA; 3 animals per nursing mother; n=24). Body weight was measured on the 3rd, 7th, 14th, 21st and 30th day of postnatal life. Food consumption during mastication was performed based on the difference in the initial and final weight of the feed offered. The analysis of chewing through filming was performed on the 22nd and 29th days of postnatal life. On the 30th day of life, animals (C; n=16; SA, n=16) were sacrificed by decapitation, where white adipose tissue was dissected and weighed. And then also at 30 days of life, transcardial perfusion was performed in other animals (C; n=8; SA, n=8), where the animal's brain was removed to perform immunohistochemistry in order to quantify SERT neurons in the mesencephalic trigeminal nucleus later on. In our results, the overfed group presented higher body weight, from the fourteenth day of life onwards (median=33.18, IIQ=34.73-30.92. p<0.001), higher amounts of inguinal fat (mean± sd=0.855±0.158, p<0.001), epididymal (mean ± sd= 0.120±0.046, p<0.001), mesenteric (median= 0.879, IIQ=1.142-0.722, p<0.001) and retroperitoneal (mean ± sd=0.106 ±0.05, p=0.017), as well as higher food consumption during mastication at 22 postnatal days (median=0.665, IIQ=0.825-0.490, p=0.018). The overfed group also had a lower number of chewing sequences, as well as fewer chewing cycles and a lower chewing rate. In addition, there was a lower expression of SERT-IR neurons in the mesencephalic trigeminal nucleus, when compared to the control group. Thus, we conclude that rats with obesity induced by neonatal supernutrition have higher body weight, higher amounts of white adipose tissue, higher food consumption during mastication, lower rhythmic masticatory activity and lower SERT expression. We hope that the findings of this study will help in the development of effective therapeutic strategies for the control and reduction of obesity.

COMMITTEE MEMBERS:
Presidente - 2447615 - LIGIA CRISTINA MONTEIRO GALINDO
Externa à Instituição - MARCIANE MILANSKI FERREIRA
Interna - 2934792 - RAQUEL DA SILVA ARAGAO