Banca de DEFESA: LETÍCIA DA SILVA PACHÊCO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LETÍCIA DA SILVA PACHÊCO
DATE: 28/02/2023
TIME: 14:00
LOCAL: GOOGLE MEET
TITLE:
KEY WORDS:

Maternal obesogenic diet; Gestation; Lactation; hepatic metabolism; Liver; Offspring.

PAGES: 50
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

In recent decades, the consumption of calorie-dense foods with low nutritional value has been increasing. Epidemiological and experimental studies have shown that the ingestion of an obesogenic maternal diet is related to an increased risk of developing metabolic disorders in the offspring. The objective was to evaluate the repercussions of the maternal obesogenic diet, during pregnancy and lactation, on the activity and quantification of mRNA of energy metabolism enzymes in the liver of the offspring, at 30 and 90 days. Pregnant Wistar rats were divided into a control group (C) (n=8) that received a standard animal house diet and an obesogenic group (OB) (n=8) that received an obesogenic diet, consisting of a high fat diet plus the offer of condensed milk during pregnancy and lactation. After weaning, the offspring received a standard animal house diet until reaching 30 and 90 days of age, when they were euthanized. In mothers, food consumption, body and liver weight were evaluated. In the offspring, body and liver weight, waist circumference, naso-anal length, Lee index, activity of the metabolic enzymes citrate synthase (CS) and β-hydroxyacyl-CoA dehydrogenase (β-Had) and gene expression of CS were verified. and AMPK. Our results show that the body weight of mothers during pregnancy was higher in the OB group than in the C group (OB:101.2±5.932 vs C:66.44±4.857g, p=0.0003). During lactation, the body weight of the OB group was reduced compared to C (OB:27.50±2,262 vs C:37.67±3,040g, p=0.0230). Food consumption, considering the total period, during pregnancy the OB group consumed less protein (OB:246.0±14.23 vs C:550.6±43.39Kcal, p=0.0002) and more lipids than the C group (OB:420.4±17.61 vs C :213.1±16.79Kcal, p<0.0001). During lactation, the OB group compared to the C group showed a reduction in the consumption of proteins (OB:392.0±42.71 vs C:1025±19.53Kcal, p<0.0001) and carbohydrates (OB: 1410±108.4 vs C:2201±41.95Kcal, p =0.0001), however, higher consumption of lipids (OB:674.7±50.52 vs C:396.6±7.55Kcal, p=0.0006) and total calories from macronutrients (OB:2477±194.0 vs C:3623±69.03Kcal, p=0.0005 ). In mothers, liver weight, carbohydrate consumption and total Kcal did not show statistical difference between the groups surveyed. In the offspring, body weight increased in the OB group compared to C at 30 days (OB:99.11±3.048 vs C:90.67±1.810g, p=0.0300), at 90 days the OB showed a decrease compared to C (OB:308.0±8.658 vs C:351.2±7.141g, p=0.0015). Abdominal circumference at 30 days increased in the OB group compared to C (OB: 12.44±0.2422 vs C:11.79±0.1487cm p=0.0491). The Lee index at 30 (OB:297.8±4.917 vs C:284.7±2300g/cm³, p=0.0353) and 90 days (OB:300.6±3225 vs C:286.5±2384 g/cm³, p=0.0044) demonstrated increase in OB offspring compared to their respective controls. The enzymatic activity of CS showed an increase in the OB group compared to the C group at 30 (OB:0.0185±0.0013 vs C:0.0104±0.0017 U/mg) and 90 days (OB:0.0074±0.0005 vs C:0.0048±0.0005U/mg) . β-Had showed a decrease in activity in the OB group compared to C with 30 (OB:0.4721±0.01820 vs C:0.6492±0.1072 nmol/min/mg) and 90 days (OB:0.3182±0.0647 vs C:1.040± 0.2337nmol/min/mg). In offspring, liver weight, naso-anal length, waist circumference at 90 days and gene expression of AMPK and CS at 30 days did not show significant difference between the groups analyzed. Preliminary data suggest that maternal

COMMITTEE MEMBERS:
Interna - 2063134 - ALICE VALENCA ARAUJO
Externa à Instituição - TALITTA RICARLLY LOPES DE ARRUDA LIMA
Presidente - 3137867 - VIVIANE DE OLIVEIRA NOGUEIRA SOUZA