Banca de QUALIFICAÇÃO: CYNTHYA MYLLENA MARTINS SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : CYNTHYA MYLLENA MARTINS SILVA
DATE: 29/06/2022
LOCAL: GOOGLE MEET
TITLE:
KEY WORDS:

Obesity; Serotonin; Chewing; Litter Size.

PAGES: 43
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

The increase in body weight may be associated with changes in masticatory function. In overweight and obese conditions, energy balance control is clearly dysregulated and serotonergic modulation appears to significantly contribute to weight gain. This study aims to evaluate the effect of obesity induced by reduced litter size on somatic and masticatory parameters and serotonin transporter immunoreactivity (SERT) in masticatory trigeminal nuclei. The study, carried out with Wistar rats, was submitted and approved by the Ethics Committee on Animal Experimentation of the Federal University of Pernambuco (0026/2019). Rats were mated at a ratio of 2 females to 1 male and maintained in standard vivarium conditions. Thirty-two male Wistar albino rats (Rattus Norvegicus) from female rats from the vivarium colony created by the Nutrition Department of the Federal University of Pernambuco were used. Seventy-two hours after birth, the animals were randomly divided into two groups: Control (C; 9 pups per nursing mother; n=16) or superfed (SA; 3 animals per nursing mother; n=16). Body weight was measured on the 3rd, 7th, 14th, 21st and 30th day of postnatal life. From the difference in the initial and final weight of the ration, the difference was calculated and food consumption was analyzed. The analysis of mastication through filming was performed on the 22nd and 29th days of postnatal life. On the 30th day of life, the animals were sacrificed by decapitation, where white and brown adipose tissue was dissected and weighed, and transcardiac perfusion was performed, where the animal's brain was removed for immunohistochemistry later on. results, the overfed group had higher body weight, from the fourteenth day of life (median=33.18, IIQ=34.73-30.92. p<0.001), higher amounts of inguinal fat (mean± SD= 0.855±0.158, p<0.001), epididymal (mean±sd=0.120±0.046, p<0.001), mesenteric (median=0.879, IIQ=1.142-0.722, p<0.001) and retroperitoneal (mean±sd=0.106±0 .05, p=0.017), as well as higher food consumption at 22 postnatal days (median=0.665, IIQ=0.825-0.490, p=0.018). Thus, we conclude that rats with obesity induced by neonatal overnutrition have higher body weight, higher amounts of white adipose tissue and higher food consumption. We hope that the findings of this study will help in the development of effective therapeutic strategies for the control and reduction of obesity.

BANKING MEMBERS:
Presidente - 1802315 - CLAUDIA JACQUES LAGRANHA
Externo à Instituição - DIEGO CABRAL LACERDA
Interna - 2447615 - LIGIA CRISTINA MONTEIRO GALINDO