Banca de DEFESA: KELITON GOMES DE SOUZA CRUZ

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : KELITON GOMES DE SOUZA CRUZ
DATE: 07/08/2024
TIME: 13:00
LOCAL: Programa de Pós-Graduação em Ciência de Materiais
TITLE:

Carbonaceous nanozymes and study of their peroxidase activity

KEY WORDS:

Nanoparticles, carbon dots, nanoenzymes, catalytic activity.

PAGES: 64
BIG AREA: Outra
AREA: Multidisciplinar
SUMMARY:

Nanomaterials are structures that are in the order of up to 100 nm in size and have physicochemical properties that differ from larger-scale materials. Among nanometric materials, carbon-based materials, such as carbon dots, are widely trained due to their biocompatibility, low toxicity in biological systems, low cost and in many cases, they are easy to functionalize. Due to the wide applicability, these materials can be used as biomarkers, biosensors, drug carriers, enzymes, among other applications. Therefore, this research proposes to use carbon dots synthesized from bovine serum albumin (BSA) to be applied as a peroxidase mimetic enzyme in contact lenses. The enzyme peroxidase is found in several organisms, such as plants, bacteria and humans, and its function is to break the bonds of hydrogen peroxide (H2O2) to produce oxygen. In this work, NP, NPFe and NPMn nanoparticles were synthesized from BSA, with the NPFe nanoparticle being doped with iron, while the NPMn nanoparticle was doped with manganese. Reaction kinetic studies showed that NP and NPFe nanoparticles presented the best performance in terms of reaction speed and Km, with NPFe being the best of the 3 nanoparticles. NPMn presented the worst performance and at concentrations considered low it does not present any enzymatic activity. The experiments and graphic data obtained show that the NPFe nanoparticle presented a Km = 6,53 mmol.L-1 and Vmax = 5,33x10-6 mol.s-1, the NP presented Km = 21.37 mmol.L-1 and Vmax = 5.77x10-5 mol.s-1 and NPMn presented Km = 131,45 mmol.L-1 and Vmax = 7,37x10-4 mol.s-1. These results suggest that NP nanoparticles and especially NPFe have great potential to be applied with peroxidase function, and can be applied to contact lenses.

COMMITTEE MEMBERS:
Interno - 1167723 - ARMANDO JUAN NAVARRO VAZQUEZ
Presidente - 1963108 - JORGE LUIZ NEVES
Externa à Instituição - THAIS MEIRA MENEZES - UFRPE