Dissertations/Thesis

Clique aqui para acessar os arquivos diretamente da Biblioteca Digital de Teses e Dissertações da UFPE

2024
Dissertations
1
  • AUDICLERE DE SOUSA SILVA
  • Impact of Self-Collection Assessment on Tracking and Detection of
    Cervical Cancer

  • Advisor : JACINTO DA COSTA SILVA NETO
  • COMMITTEE MEMBERS :
  • IVONE ANTONIA DE SOUZA
  • MARIO RIBEIRO DE MELO JUNIOR
  • BRUNO MENDES TENORIO
  • Data: Jan 29, 2024


  • Show Abstract
  • Cervical cancer is the fourth most common neoplasm among women globally. Cytology is the
    primary screening method, but molecular advancements in HPV detection allow for the distinction
    of oncogenic types. This study evaluated the efficacy of cervicovaginal self-collection in cervical
    cancer screening, comparing it with conventional cytology. With 404 women (age > 18 years),
    23% with a secondary education level and 74.5% with higher education, self-collection was
    analyzed for safety and adherence. The observational and analytical cross-sectional method
    collected data in Recife and Cabo de Santo Agostinho. Using Papanicolaou and the Bethesda
    System, the analysis revealed significant associations in age groups (p = 0.036) and atypical
    microbiota (p = 0.001). Conventional oncologic cytology showed higher positivity in nulliparous
    women (p = 0.015). The presence of neoplastic cells was significant in non-menopausal women (p
    = 0.022). Logistic regression indicated significance between secondary and higher education (p =
    0.023) and smoking (p = 0.018). The study, in addition to cervical profiles and relationships,
    highlighted 79.9% approval for self-collection, contributing to strategies for cervical cancer
    prevention and improving the quality of life for women in Pernambuco.
2
  • VERA KAISSA SOUZA SANTOS BACELAR
  • HISTOPATHOLOGICAL EVALUATION AND DETERMINATION OF EXPRESSION OF SURFACTANT PROTEIN TYPE-B (SP-B) IN THE LUNGS OF SENTIC RATS TREATED WITH CEFTRIAXONE AND ANTIOXIDANTS.

  • Advisor : MARIA BERNADETE DE SOUSA MAIA
  • COMMITTEE MEMBERS :
  • ERYVELTON DE SOUZA FRANCO
  • MARIO RIBEIRO DE MELO JUNIOR
  • ANDRE MARTINS GALVAO
  • Data: Jan 30, 2024


  • Show Abstract

  • Introduction: Sepsis is defined as a potentially fatal organ dysfunction caused by an insufficient response in controlling the infectious agents involved. Due to the increasing number of cases, high mortality rate and high cost of treating sepsis, it has become a public health problem. Sepsis is among the main diseases involved in the onset of acute lung injury (ALI), since the lung is one of the organs most commonly affected. Objective: Histopathological evaluation and determination of surfactant protein type-b (sp-b) expression in the lungs of septic rats treated with ceftriaxone and antioxidants. Methodology: A total of 70 male Wistar rats (200-350g) were used to compose seven experimental groups (n=10/group). Of these, 40 were submitted to induction of sepsis by Cecal Ligation and Puncture (CLP) and subdivided into the treated: I) Distilled water (5 ml/kg; i.p.) = CLP+AD; II) ceftriaxone (30 mg/kg; i.p.) = CLP+ATB; III) antioxidants (A. Ascorbic acid 50mg/kg, NAC 10mg/kg and Alpha tocopherol 20mg/kg; i.p) = CLP+ATO; IV) ceftrianone (30 mg/kg; i.p.) and antioxidants (Ascorbic A 50mg/kg, NAC 10mg/kg and alpha tocopherol 20mg/kg; i.p) = CLP+ATO+ATB. The SHAM group (control) was composed of three groups (n=10/group), which underwent the same surgical procedure without inducing sepsis and were treated in the same way as CLP: I) SHAM+DA; II) SHAM+ATB; III) SHAM+ATB+ATO. After 24 hours of the procedure, the animals received the treatment for five consecutive days. During this period, the animals were observed for functional/physiological characteristics and, at the end, they were anesthetized for blood collection, determination of the hematological profile and then euthanized by anesthetic deepening to collect the left lower lung lobe for histomorphometric evaluation (quantification of neutrophils, macrophages , lymphocytes, type II pneumocytes, alveoli and 'determination of alveolar area and thickness of alveolar septa) and determination of SP-B protein expression by Western Blot. Results: The percentage of experimental mortality in the septic group was 21.42%, which made it possible to continue the groups with n=6. The survival rate of the CLP groups varied significantly (P<0.05) according to the type of treatment and observation time: CLP+AD (48h – 50.67%; 72h – 50.67%; 84h – 50, 67% and 96h – 50.67%); CLP+ACT (84h – 68% and 96h – 68%); CLP+ATB (48h - 34%; 72h – 34%; 84h – 34% and 96h – 18%) when compared to the SHAM+AD group at the same times. As for the physiological characteristics, the septic animals treated with ATO or ATB+ATO showed a significant improvement (P<0.05) in the condition of mobility, lethargy and diarrhea compared to the CLP+AD group. In the hematological evaluation, the platelet count (103/dL) was significantly reduced (P<0.05) in the CLP+ATB group (331.33±131) and this decrease was not verified in the CLP+ATB groups (627± 25.32) and CLP+ATB+ATO (437.33± 97.88) when compared to SHAM+AD (429± 69). As for histomorphometry, it was verified that the infiltration of inflammatory cells in the lung tissue of animals treated with ATO or ATB+ATO did not present a significant difference compared to the SHAM+AD group, on the other hand, animals treated with CLP+AD or ATB showed a significant increase (P<0.05) of inflammatory cells compared to that observed in SHAM+AD. Regarding the number of type II pneumocytes, the groups treated with ATO or ATB+ATO showed no significant difference compared to SHAM+AD. However, the one treated with ATB alone significantly reduced the amount of type II pneumocytes in this group compared to all others. The alveolar quantity in the CLP animals treated with ATO alone were similar to those verified in the SHAM+AD and CLP+ATB+ATO groups. The septic animals treated with ATB had a greater number of alveoli, but with a smaller alveolar area compared to the other groups. In turn, the alveolar area in the septic groups treated with ATB; ATO or ATB+ATO exhibit significantly smaller area compared to SHAM+AD. With regard to the thickness of the alveolar septa of animals treated with ATO or ATB+ATO, these were similar to SHAM+AD. This fact was not verified in septic animals treated with ATB alone, which presented septal thickness greater than those observed in animals treated with ATO or ATO+ATB. Conclusion: Given the above, antioxidants (vitamin C, vitamin E and N-acetylcysteine) when administered intraperitoneally in an animal model of sepsis, associated or not with an antibiotic, seem to promote the conservation of the histological structures present in the pulmonary matrix even when subjected to injuries resulting from this infectious disease, and increases the survival of animals that received these treatments.

3
  • JULLIANO MATHEUS DE LIMA MAUX
  • PERFIL INFECTIVO PARA DNA-HPV EM AMOSTRAS DE LAVADOS BRONCOALVEOLARES EM PACIENTES DA REGIÃO METROPOLITANA DO RECIFE, PERNAMBUCO-BRASIL

  • Advisor : JACINTO DA COSTA SILVA NETO
  • COMMITTEE MEMBERS :
  • BIANCA DE FRANCA SAO MARCOS
  • MARIO RIBEIRO DE MELO JUNIOR
  • VANESSA EMANUELLE PEREIRA SANTOS
  • Data: Mar 26, 2024


  • Show Abstract
  • As doenças pulmonares são grandes causadoras de morbimortalidade a nível mundial, seja por caráter oncológico ou não oncológico. Dentre as doenças que acometem o tecido pulmonar, as Doenças Intersticiais Pulmonares (DIP) ganham notoriedade clínica, pois apresentam natureza complexa e sendo necessário a atuação interdisciplinar para diagnosticá-las. Além disso, as DIPs podem ser classificadas quanto a seus fatores etiológicos (secundária ou idiopática), grau de inflamação (agudo ou crônico) e pela população de células imunológicas encontradas no tecido. Para investigar suspeitas de DIPs, é utilizado o lavado broncoalveolar (LBA) para recuperar células imunes no interstício pulmonar. Os parâmetros citológicos encontrados no fluido do LBA podem sugerir alterações compatíveis com várias doenças pulmonares. Não obstante, o LBA também pode ser utilizado para pesquisas microbiológicas e moleculares. Diversos vírus que provocam alterações pulmonares podem ser encontrados no LBA, contudo, não há pesquisas que englobem o Papilomavirus Humano (HPV) como agente etiológico de doenças pulmonares, mesmo que seu DNA já foi encontrado em tecidos não ginecológicos, incluindo pulmão. O presente estudo busca detectar o DNA-HPV no trato respiratório inferior por intermédio do LBA, e associá-lo como agente etiológico de doenças pulmonares, além de relacionar com os achados citológicos e possíveis efeitos citopáticos. Para isso, foi conduzido um estudo observacional, analítico transversal. Um total de 106 pacientes, 63 mulheres (59,46%) e 43 homens (40,56%), de hospitais da Região Metropolitana de Recife foram inclusos (média de idade 52 anos). Entre esses, 10 (9,43%) são tabagistas, 33 (31,13%) são ex-tabagista e 54 (50,94%) alegam nunca ter fumado. Dentre as principais hipóteses clínicas, 19 (17,92%) dos pacientes tinham suspeita de neoplasia ou não apresentam suspeitas concretas. Já as suspeitas de infecções, pneumonia de hipersensibilidade e doenças pulmonares de causas diversas apresentaram 18 (16,98%), 17 (16,30%) e 8 (7,54%), respectivamente. Dos 106 pacientes, 103 (97,16%) LBAs foram considerados satisfatória para análise. A linfocitose foi presente em 28 (26,41%), a neutrofilia em 92 (86,79%) e a eosinofilia em 17 (16,03%) das ocorrências. Casos de linfocitose e neutrofilia foram encontrados em 23 (21,69%) dos lavados analisados, sugerindo que a citologia quantitativa possui melhores valores preditivos quando associados a exames clínicos e de imagens. Além disso, é fundamental relacionar com as características citomorfológicas, principalmente dos macrófagos alveolares e das células epiteliais, resultando em um laudo citológico fidedigno. 

2023
Dissertations
1
  • HIAGO HENRIQUE BEZERRA NUNES
  • APPLICATION OF RECURRENCE ANALYSIS FOR DETECTION OF MYOCARDIAL INFARCTION ON 12 LEAD ECG SIGNALS


  • Advisor : FERNANDO JOSE RIBEIRO SALES
  • COMMITTEE MEMBERS :
  • MARILU GOMES NETTO MONTE DA SILVA
  • PAULO SALGADO GOMES DE MATTOS NETO
  • TIAGO PAGGI DE ALMEIDA
  • Data: Jan 27, 2023


  • Show Abstract
  • The use of nonlinear dynamic systems analysis methods to identify cardiac arrhythmias from electrocardiogram (ECG) signals has become widely disseminated, especially the technique of recurrence quantification analysis (RQA) of reconstructed phase space (PS). However, the choice of PS reconstruction parameters, delay and dimension, is still controversial in the literature. The main objective of this study is to evaluate the impact of the choice of delay and dimension on the classification of ECG signals in healthy patients and patients with myocardial infarction (MI). The classification was executed by producing a k-nearest neighbors (KNN) model trained from RQA attributes taken from two forms of PS reconstruction, one with optimal embedding parameters calculated for each signal and another with fixed embedding parameters. Complementally, the performances of the algorithm to detect MI in different affected cardiac regions were also evaluated, besides testing five different techniques of attribute normalization. The results indicate that despite the models with fixed embedding parameters, in general, having superior performance to the models with variable embedding parameters, rarely this difference was statistically significant. When the infarction location is taken into account, among the best results, the performance of the KNN model with fixed embedding parameters was superior in all cases where there was a statistically significant difference. In addition, we obtained a score of 0.815 on the ROC AUC for MI detection and the MinMax scaler was the most robust attribute normalization technique.

2
  • CAROLINA DIAS DA SILVA AMORIM
  • EVALUATION OF CLINICAL IMPACT OF INTERLEUKIN 8 GENE EXPRESSION IN PATIENTS WITH FIBROMYALGIA.

  • Advisor : CLAUDIA DINIZ LOPES MARQUES
  • COMMITTEE MEMBERS :
  • ALINE RANZOLIN
  • NARA GUALBERTO CAVALCANTI
  • RICARDO OLIVEIRA DA SILVA
  • Data: Jun 21, 2023
    Ata de defesa assinada:


  • Show Abstract
  • Fibromyalgia (FM) is a musculoskeletal syndrome characterized by diffuse and chronic pain associated with other symptoms such as fatigue, sleep/cognition disorders, headache, depression, and anxiety, resulting from a change in pain processing. Some working has shown an increase in some interleukins (IL1, IL6, IL8, IL10, IL17 and TNF- α) in patients with FM when compared to controls, however, there is still no uniformity and consensus in the results. It is known that patients with FM have a higher frequency of obesity compared to the general population and recent studies have shown an increase in interleukin 8 (IL8) in patients with obesity as well as in FM. There is no study evaluating IL8 in FM and association with obesity and other clinical parameters. To verify the impact of IL8 gene expression on the clinical parameters of patients with FM and compare whether IL8 gene expression is increased in relation to the comparison group (CG). Cross-sectional, observational, descriptive and analytical study, carried out from August to December 2021, with the sample of cases consisting of patients diagnosed with FM (GFM) treated at the rheumatology service of the Hospital das Clínicas of the Federal University of Pernambuco (HC-UFPE). The CG was composed of patients' companions and hospital staff. Clinical and demographic data were collected in both groups, in addition to weight and height for calculating the Body Mass Index (BMI), application of questionnaires for: fatigue (Functional assessment of chronic illness therapy), depression (Beck depression inventory), sleep (numerical sleep scale) and impact of FM (Fibromyalgia Impact Questionnaire Revised) and peripheral blood collection for evaluation of IL8 gene expression analyzed through the difference between the target gene (IL8) and the endogenous control gene (B-actin). Study variables were: expression of IL8, sex, age, race, physical activity, duration of illness, occupational situation, comorbidities, use of medications, values of questionnaires for fatigue, sleep, depression and impact of fibromyalgia. Patients with FM did not show a higher frequency of IL8 gene expression compared to the CG, but among individuals who express IL8, the FM group had a higher gene expression. There was no association of IL8 with worse FIQ-R indices, sleep disturbance, BMI or fatigue, but there was an association with depression, where expressing IL8 showed worse indices in BDI (p= 0.002). Patients with FM do not have a higher frequency of IL8 expression compared to CG and patients with IL8 expression
    have a greater association with moderate depression than patients who do not express IL8.

3
  • JAMAICA GINA ELOI DE SOUZA GUIMARÃES
  • Association between Th1 and Th2 responses and the evolution of severe acute respiratory syndrome by SarsCov2.

  • Advisor : PAULO SERGIO RAMOS DE ARAUJO
  • COMMITTEE MEMBERS :
  • JOSE ANGELO RIZZO
  • LIDIER ROBERTA MORAES NOGUEIRA
  • FABIANNE MAISA DE NOVAES ASSIS DANTAS
  • Data: Jun 22, 2023
    Ata de defesa assinada:


  • Show Abstract
  • Cytokine storm is being considered as one of the main mechanisms contributing to acute lung injury and the development of COVID-19. In view of the fundamental role that cytokines seem to play in the pathophysiology of COVID-19, it is essential to analyze and better understand the underlying factors that influence the worsening of the disease. In this context, the study aimed to analyze the profiles of inflammatory and anti-inflammatory cytokines and the evolution of severe acute respiratory syndrome due to COVID-19. This is a descriptive, quantitative, observational, retrospective, cross-sectional cohort study involving 167 patients who were admitted to the Intensive Care Unit or ward with severe acute respiratory syndrome due to COVID-19. Data referring to sociodemographic, clinical and laboratory information were collected from patients' medical records and tabulated in a spreadsheet. For data analysis, SPSS 13.0 software (Statistical Package for the Social Sciences) for Windows and Microsoft Excel 365 were used. There was a statistically significant difference in the levels of cytokines IFN-Y, IL-10 and IL-6 in relation to the moments analyzed. There was also a relationship between cytokine levels, especially IL-6 and IL-10, with the degree of pulmonary impairment, need for mechanical ventilation, length of hospital stay and death. There was a significant relationship between elevated levels of inflammatory and anti-inflammatory cytokines and SARS severity, especially in relation to IL-6 and IL-10 levels.

4
  • EMANUEL FERNANDES FERREIRA DA SILVA JUNIOR
  • ATENUAÇÃO DE RUÍDO EM SINAIS ELETROMIOGRÁFICOS DE SUPERFÍCIE EM MÚSCULOS RESPIRATÓRIOS PARA MONITORAMENTO DURANTE OXIGENOTERAPIA EM PACIENTES COM COVID-19 NA UNIDADE DE TERAPIA INTENSIVA


  • Advisor : MARCELO RENATO GUERINO
  • COMMITTEE MEMBERS :
  • CATARINA SOUZA FERREIRA RATTES LIMA
  • MARIA DAS GRACAS RODRIGUES DE ARAUJO
  • MARILU GOMES NETTO MONTE DA SILVA
  • Data: Aug 30, 2023
    Ata de defesa assinada:


  • Show Abstract
  • Introdução: A Eletromiografia de superfície (EMGs) caracteriza-se por ser uma ferramenta de fácil aplicação e sensível para captação de atividades musculares. Objetivo: Analisar o uso de filtros digitais para melhor condicionamento dos sinais de EMGs, a fim de monitorar os músculos respiratórios em pacientes em oxigenoterapia com COVID-19 internados na Unidade de Terapia Intensiva (UTI). Metodologia: Analisamos uma coorte de pacientes com COVID-19 submetidos à análise de sEMG respiratória em uso de oxigenoterapia de baixo fluxo, sob registro do clinical trials (NCT05572853). Foram desenvolvidas estratégias para melhor condicionamento dos sinais, em conjuntos de filtros (filtro Notch de 60Hz e filtro passa-banda entre 20-500Hz) através do Software (Scilab 6.1.1). Todos os registros mioelétricos respiratórios foram separados em dois momentos: pré-filtro e pós-filtro, analisados por meio da RMS e EMGmax do esternocleidomastóideo, escalenos, diafragma e reto abdominal. Resultados: Foram incluídos 10 pacientes com idade de 45.3 ± 6.1 anos, sendo 8 do sexo masculino. Ao comparar os valores de RMS e EMGmax em todos os músculos respiratórios avaliados, observamos reduções significativas nos sinais (P < 0.001*). Demonstrados em maiores valores para menores da RMS nos músculos avaliados pré-filtro e pós-filtro, observamos divergências no 2º músculo mais acionado, tornando estes pré-filtro: diafragma e pós-filtro: esternocleidomastóideo. Conclusão: Visando explorar com excelência o drive neural respiratório por meio de sEMG e pretendendo analisar dados qualitativamente confiáveis, observamos que o uso de filtros digitais repercute no melhor condicionamento dos sinais respiratórios de sEMG na UTI.


5
  • DENISE MAIARA DA SILVA CARNEIRO
  • MONITORING OF CHILD AND TEENS’ ADHESION TO ALLERGIC RHINITIS PROPHYLAXIS BY NMR-BASED METABONOMICS ASSAYS

  • Advisor : RICARDO OLIVEIRA DA SILVA
  • COMMITTEE MEMBERS :
  • MARIO RIBEIRO DE MELO JUNIOR
  • ANA CAROLINE CAVALCANTI DELA BIANCA MELO
  • EDIL DE ALBUQUERQUE RODRIGUES FILHO
  • Data: Oct 18, 2023


  • Show Abstract
  • Allergic rhinitis is an immunoglobulin E mediated chronic inflammatory disease that affects about 40% of world population, being very frequent among children and teenagers. The effectiveness of allergic rhinitis treatment is dependent on patients’ adherence to therapy. In clinical practice, this patients’ adherence assessment is subjective, once uses patient self-report. In this paper, we report a pilot study using NMR-based Metabonomics to monitor childrenand teenagers’ adherence to allergic rhinitis treatment. This study was developed at Clinics Hospital of UFPE, Brazil.Twenty-five volunteers were included in the study. 1H NMR spectra of serum these volunteers were obtained and processed using multivariate statistical tools. Initially, Hierarchical Clusters Analysis (HCA) was employed for spontaneous grouping of the samples. Two groups were defined containing eleven and fourteen samples each. It was not observed relationship among patients’ self-report of adherence and the groups identified by HCA. Main difference observed between the groups was the response to treatment – nine of eleven volunteers did not response to treatment, while six of fourteen volunteers did not responseto treatment in another group. Orthogonal Partial Least Square Discriminant Analysis (OPLS-DA) was used to identify the main variables responsible for discrimination. Alanine, serine, arginine, choline, N-acetyl D-glucosamine, glutamate, lactate, glucose, VLDL/LDL were the metabolites identified. The metabolites that had high serum level among those volunteers who did not response to treatment are associate with the chronicity and severity more intense of disease than those volunteers who presented some response to treatment. This is an indication that NMR based metabonomics can be useful to monitor allergic rhinitis treatment.


2022
Dissertations
1
  • PAULA ARARUNA BERTAO
  • ASSOCIATION BETWEEN CARDIOTOXICITY RISK AND ELECTROCARDIOGRAPHIC FINDINGS IN PATIENTS SUBMITTED TO ANTHRACYCLINE CHEMOTHERAPY.

  • Advisor : BRIVALDO MARKMAN FILHO
  • COMMITTEE MEMBERS :
  • MARCELO RENATO GUERINO
  • SANDRO GONCALVES DE LIMA
  • SIMONE CRISTINA SOARES BRANDAO
  • Data: Jul 19, 2022


  • Show Abstract
  • Introduction: Anthracycline chemotherapy treatment is associated with cardiotoxicity. Echocardiography is a useful tool in the detection of early myocardial injury. The electrocardiogram still has no established role in this scenario. Objective: To analyze the association between electrocardiographic changes and the development of subclinical cardiotoxicity in cancer patients undergoing treatment with anthracycline. Methods: Prospective cohort of cancer patients treated with anthracycline. Transthoracic echocardiograms with global longitudinal strain and electrocardiogram were performed before the start of treatment, at 3 and 6 months of follow-up to assess the presence or absence of subclinical cardiotoxicity, defined by a relative drop in global longitudinal strain greater than 15% in relation to the initial measurement. on at least one echocardiogram. Fisher's exact test was applied to assess this association. Results: Of the 33 patients included (mean age = 49 ± 9.5 years), 23 had breast cancer. Eight (24.24%) had incipient cardiotoxicity. QTc in women was higher than baseline at 6 months by both Bazett and Fridericia (p = 0.011 and p =0.038, respectively). There was an association, in women, between QTc > 460ms (corrected by Bazett) at 3 months and a drop in global longitudinal strain >15% in this period (p=0.043). Conclusion: The presence of QTc > 460ms at 3 months in women was associated with subclinical cardiotoxicity.

2
  • LARISSA CAROLINE DE ALMEIDA SOUSA LIMA
  • EVALUATION OF THE EFFECT OF A BIOPRODUCT OBTAINED FROM Parkinsonia aculeata ON COMPONENTS OF THE RENIN-ANGIOTENSIN SYSTEM (RAS) IN THE RENAL TISSUE OF RATS WITH EVIDENCE OF METABOLIC SYNDROME


  • Advisor : MARIA BERNADETE DE SOUSA MAIA
  • COMMITTEE MEMBERS :
  • GUSTAVO PINA GODOY
  • BELMIRA LARA DA SILVEIRA ANDRADE DA COSTA
  • ERYVELTON DE SOUZA FRANCO
  • Data: Aug 24, 2022
    Ata de defesa assinada:


  • Show Abstract
  • Obesity is recognized as a global public health problem, associated with the development of several non-communicable chronic diseases that can cause disorders and alterations such as those observed in metabolic syndrome (SM). Thus, the objective was to evaluate the effect of the polar fraction obtained from the aerial parts of Parkinsonia aculeata (PfrHEPA), the expression of the AT1 receptor for angiotensin II in the renal tissue in an experimental model of SM. For SM induction, 24 male Wistar rats were fed a Western diet (DO) for 150 days, divided into 4 groups (n=6) and the control group (n=6) was fed a standard diet (SD). During the 150 days, the animals were monitored weekly for weight gain, and at the end of this period, the systemic blood pressure (SBP) was evaluated and blood was collected to evaluate the lipid, glycemic and hematological profile, to verify the establishment of SM in these animals. The MS groups maintained their DO and were treated for 30 consecutive days with PfrHEPA 70mg/kg, PfrHEPA 140mg/kg), metformin 500mg/kg or vehicle 10mL/kg, orally. At the end of treatment, PAS was determined and blood was collected for biochemical and hematological evaluation. Subsequently, the animals were euthanized under anesthetic depth to collect the renal tissue in order to analyze the expression of the AT1 receptor for angiotensin II by means of immunohistochemistry. It was evidenced that all animals submitted to DO developed SM, presenting obesity, dyslipidemia, hyperglycemia and arterial hypertension. After treatment, the animals that received PfrHEPA (70mg/kg or 140mg/kg) showed a significant reduction in serum levels of total cholesterol (22% and 32%); triglycerides (44% and 51%) and blood glucose (27% and 43%) respectively; in relation to blood pressure levels, treatments at the same doses reduced by 22% and 24%, respectively. This result is in line with the immunohistochemical findings, where there was a reduction in the expression of the AT1 type receptor of angiotensin II in the glomerulus and renal tubule of the treated animals. In the histopathological analysis of the renal tissue, no morphostructural changes were observed. Thus, it is concluded that PfrHEPA, regardless of the doses tested, was able to reduce the expression of the AT1 receptor and modulate blood pressure in Wistar rats with SM.


3
  • DIEGO RAFAEL FREITAS BERENGUER
  • 18F-FDG PET/CT IN THE EARLY DIAGNOSIS OF CARDIOTOXICITY: EVALUATION OF THE BEST SITE TO OBTAIN THE CARDIAC SUV INDEX

  • Advisor : BRIVALDO MARKMAN FILHO
  • COMMITTEE MEMBERS :
  • DINALDO CAVALCANTI DE OLIVEIRA
  • ANDREA BEZERRA DE MELO DA SILVEIRA LORDSLEEM
  • MARIA INES REMIGIO DE AGUIAR
  • Data: Aug 26, 2022


  • Show Abstract
  • The diagnosis of cardiotoxicity has been a challenge in the daily routine of the oncologist. Today's diagnostic methods are not capable of detecting this clinical condition early enough. In this context, studies indicate that 18F-FDG PET/CT is a complementary test that can identify cardiotoxicity earlier. In this way, cardioprotection measures can be started earlier, improving the chances of increasing patient survival. Objective: To evaluate the behavior of the standardized uptake value (SUV) of the radiopharmaceutical 18F-FDG before, during and after chemotherapy (QT) at different cardiac sites, as well as to measure the degree of reproducibility for the method in the context of cancer treatment follow-up. Materials and Methods: Retrospective cohort including lymphoma patients who underwent 18F-FDG PET/CT before, during and/or after chemotherapy. The uptake behavior through the mean and maximum SUVs was evaluated in four cardiac sites and in control sites in the aorta and liver. Twenty exams were randomized for reproducibility analysis by two examiners who were blinded to each other's results. Each one of them did the evaluation in a second moment to elucidate the intra-observer reproducibility. Results: A significant increase in SUVs was observed in all cardiac sites in the interim and final moments (post-terminus of QT) when compared with pre-QT SUVs. The left ventricular (LV) free wall was the cardiac region with the greatest increase in 18F-FDG uptake. As for the reproducibility, it was possible to verify substantial results for the reliability of the measurement of SUVs, both intra and inter-observer. Conclusions: Cardiac uptake of 18F-FDG increased along QT, with the LV free wall being the site with the greatest increase. The reproducibility analysis showed high intra- and inter-observer correlation values.

4
  • JULIANNA RIBEIRO DO VALE SILVA
  • EFFECTS OF RADIOFREQUENCY AND MICRONEEDLING ON THE QUALITY OF LIFE IN WOMEN WITH ALBA STREAKS: A RANDOMIZED BLIND CLINICAL TRIAL

  • Advisor : MARCELO RENATO GUERINO
  • COMMITTEE MEMBERS :
  • MARIO RIBEIRO DE MELO JUNIOR
  • JULIANA NETTO MAIA
  • MARIA DAS GRACAS RODRIGUES DE ARAUJO
  • Data: Aug 30, 2022


  • Show Abstract
  • The stretch marks are lesions of the dermal layer of the skin, commonly caused by a sudden stretching of the skin, being aggravated by hormonal, extrinsic and intrinsic factors. They are classified as rubras and alba, caused by an acute lesion and chronic, respectively. This study aims to verify the effects of the association of non-ablative bipolar radiofrequency with microneedling in the treatment of alba streaks. This is a blind randomized clinical trial, carried out in the electrothermophototherapy laboratory of the Federal University of Pernambuco. The sample was divided into two groups, group MICRO and RF+MICRO, performed microneedling therapy, and group radiofrequency associated with microneedling, respectively. Both groups had a total of three sessions. The evaluations were made with conventional and thermographic photographs, application of a quality-of-life questionnaire, anamnesis, physical evaluation, and a final questionnaire about the participant's vision after the end of the research. There was no significant difference between groups regarding the increase in local temperature. Pain and the final score of the dermatology quality of life questionnaire also showed no distinction. The results analyzed from conventional photographs were also not significant. Thus, there was no difference between the microneedling compared to the association of radiofrequency and microneedling in the alba streaks treatment.


5
  • LAISA WANESSA SANTOS LIMA
  • EVALUATION OF THE EFFECT OF THE POLAR FRACTION OF THE EXTRACT OF AERIAL PARTS OF Parkinsonia aculeata L. ON COMPONENTS OF THE RENIN-ANGIOTENSIN SYSTEM (RAS) AND ON MARKERS OF OXIDATIVE STRESS IN THE HEART TISSUE OF RATS WITH METABOLIC SYNDROME

  • Advisor : MARIO RIBEIRO DE MELO JUNIOR
  • COMMITTEE MEMBERS :
  • IVONE ANTONIA DE SOUZA
  • GLORIA ISOLINA BOENTE PINTO DUARTE
  • SINARA MÔNICA VITALINO DE ALMEIDA
  • Data: Nov 16, 2022


  • Show Abstract
  • Changes in eating habits and a sedentary lifestyle over the last few years have influenced the increase in obesity, considered a worldwide epidemic. The increase in energy supply through a diet rich in simple carbohydrates, lipids and low in fiber, linked to the reduction of physical activity, called contemporary western lifestyle, triggers metabolic disorders such as dyslipidemia, type 2 diabetes mellitus, cardiovascular diseases, among others. In this context, the purpose of the study was to analyze the effect of the polar fraction of the extract of aerial parts of Parkinsonia aculeata L. (PfrEHPA) on RAS components and on oxidative stress markers in the cardiac tissue of male Wistar rats after weaning ( 21 days) initially divided into two groups that received a standard diet for rodents (Presence ®) or a “Westernized” diet (OD) for 150 days. Then, these animals were divided into 5 subgroups which were treated orally for 30 consecutive days with PfrEHPA (70 or 140 mg/kg/day), metformin (500 mg/kg/day) or distilled water (10 mL/day). kg/day). During the 150 days of diet consumption, the weekly food consumption, weight evolution, body dimensions and lipid profile of the animals were recorded. These same parameters were determined again after the treatment described above. At the end of the experiment (180 days) blood samples were collected to determine the hematological and biochemical profile. Subsequently, the animals were euthanized under anesthesia and decapitation and then the organs were weighed and stored, part in formalin (10%) for later histological and immunohistochemical analysis, and part stored in a freezer with a temperature maintained at -80ºC, which was used for the analysis of oxidative extraction. Our results showed that animals submitted to the Westernized Diet showed signs of metabolic syndrome (increased waist circumference, hyperlipidemia, hyperglycemia). The change in the lipid profile of the DO group was evidenced by a significant (p<0.05) increase in serum levels of total cholesterol (91 ± 1.57), LDL (37.2 ± 2.05) and triglycerides (135, 67 ± 2.09) in relation to the PD group (64.67 ± 1.16 ; 23.67 ± 1.88 and 75 ± 4.01, respectively). These animals also showed a significant (p<0.05) increase in fasting glucose (mg/dl) compared to animals that ingested PD. The treatment with PfrEHPA (140 mg/kg), even with the continued consumption of the westernized diet by the animals (150th – 180th days), promoted a significant reduction in the levels of total cholesterol (62.33±0.80), LDL (21 .70±0.63) and triglycerides (68.16±0.79) in relation to the untreated OD group (92±0.73; 37.77±1.45 and 141.17±1.14, respectively). Additionally, treatment with PfrEHPA (140 mg/kg) also reduced serum glucose levels, systolic blood pressure and levels of oxidative stress in the cardiac tissue of these animals.

6
  • LEONORA CASTRO TENÓRIO
  • EFFECTS OF OSTEOPATHIC MANIPULATIVE TREATMENT IN THE MUSCULOSKELETIC MANIFESTATIONS OF CHRONIC PHASE CKIUNGUNYA FEVER: RANDOMIZED CLINICAL TRIAL


  • Advisor : CLAUDIA DINIZ LOPES MARQUES
  • COMMITTEE MEMBERS :
  • GUSTAVO PINA GODOY
  • ALINE RANZOLIN
  • PAULO HENRIQUE ALTRAN VEIGA
  • Data: Dec 12, 2022
    Ata de defesa assinada:


  • Show Abstract
  • The Chikungunya Fever (FC) is an epidemic illness with high morbidity rate and presents disabling arthralgia as its main clinical characteristic. Studies indicate an elevated intake of analgesic and low satisfaction during the treatment of the chronic stage. Osteopathic manipulative treatment (OMT) is a manual therapy technique, which uses mobilization and manipulation of body tissues for pain relief, improvement of musculoskeletal manifestations as well as improvement of joint functionality, presenting itself as a treatment strategy for rheumatologic diseases. The aim of this study was to evaluate the effects of OMT on the reduction of chronic musculoskeletal manifestations (pain and functional disability) in CF patients. A randomized, controlled, blind for the evaluators trial was carried out, in which 44 patients were included for analysis, divided into two groups: intervention group,which received OMT, with 24 patients; and placebo control group, with 20 patients. The osteopathy group (OG) underwent 4 sessions of OMT intervention and was reassessed within 15 days and 30 days since theirlast session, at the rheumatology clinic of the Hospital das Clínicas de Pernambuco, with protocol elaborated by the researcher. These being the inclusion criteria: men and women aged 18 years old or more; to be monitored by the CF clinic at the Hospital das Clínicas; clinical serology diagnosis for CF; present pain and rigidity in superior limbs, having more than three months of duration of the symptoms, and consenting to participate of the study. These being the exclusion criteria: patients with inflammatory joint diseases, prior to CHIKV infection; diseases that compromise functionality of the upper limbs; cognitive, auditory and visual impairment preventing the application of assessment instruments; diagnosis of neoplasia, chronic infectious diseases or other rheumatologic illnesses,and have been undergoing physical therapy treatment within the previous 2 months. The sample was obtained by spontaneous demand and recruitment. The outcome variables were: pain measured with the Visual Analog Scale (VAS), the Brief Pain Inventory Questionnaire (BPI) besides Algometer and functionality, collected with the aid of the Disabilities of the Arm, Shoulder, Hand Questionnaire (DASH) and the nine-hole peg test (9HPT). The data were analyzed quantitatively, analytically and measured in effect size. For analysis of the results, the significance level adopted was p=0.05. The average age of the sample was 53.55 (16.5) years, most of them female (95.0%). Regarding pain intensity, during the analysis of the primary endpoint, an efficacy of 77.3% was observed, the 15-day follow up showed continued improvement with efficacy of 91.5%, and 81.2% within 30 days, after the intervention with OMT, p≤0.05. In the analysis between the groups, a statistically significant reduction in pain for the OG was observed with differences in the last session (S4) = 1.2, within the 15-day follow-up (S5) = 1.84, and within 30 days (S6) = 1.45 point when compared to the control group (CG). In the intra-group analysis, when comparing the initial session to the last one and follow-ups, only the OG presented a significant statistical reduction. Regarding the secondary endpoint, of pain and functionality, it was observed an efficacy in all the evaluated instruments as well as in both 15-day and 30-day follow-ups after undergoing the OMT, except for the algometry and the 9HPT test, where there was no statistically significant improvement in both intra-group and inter-group analysis for the OG (p<0.05). It can be concluded that OMT has proved to be effective in reducing pain besides improving the functionality of patients with musculoskeletal manifestations in the chronic stage of CF.


SIGAA | Superintendência de Tecnologia da Informação (STI-UFPE) - (81) 2126-7777 | Copyright © 2006-2024 - UFRN - sigaa09.ufpe.br.sigaa09